The effect of some conditions on the secretion of extracellular beta-galactosidase synthesised by Kluyveromyces fragilis 28
Demczuk, A.; Kowalewska-Piontas, J.; Bednarski, W.
Communications in Agricultural and Applied Biological Sciences 68(2): 469-472
2003
ISSN/ISBN: 1379-1176 PMID: 24757788 Document Number: 562226
To induce extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28, it was used glycine, L-asparagine, L-leucine, dimethyl fluoride, dimethyl sulfoxide, cetyldimethylethylammonium bromide, penicillin G and glycolipids from Candida antarctica. The highest increase in the secretion of beta-galactosidase was obtained in the yeast culture cultivated in the medium with polypeptone when glycine was used as the secretion inductor. The extracellular activity of beta-galactosidase reached 0.416 A.U./ml, and was 10-fold higher than the beta-galactosidase activity reported in the control group.