Assay of 2-naphthyl beta-galactosidase activity
Asp, N.G.; Dahlqvist, A.
Analytical Biochemistry 42(1): 275-280
1971
ISSN/ISBN: 0003-2697 PMID: 5556415 Document Number: 33848
A newly synthetized substrate, 2-naphthyl β-galactoside, was tested for the assay of human small-intestinal β-galactosidase activity. The substrate is hydrolyzed exclusively by the acid β-galactosidase and can, therefore, be used for the specific assay of that enzyme. A method for assay of enzyme activity is described. Liberated 2-naphthol is detected fluorometrically.
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