Hydrolysis of lactose by immobilized beta-galactosidase
Pappel', K.E.; Siĭmer, E.Kh.; Kestner, A.I.; Letunova, E.V.; Tikhomirova, A.S.
Prikladnaia Biokhimiia i Mikrobiologiia 12(2): 217-220
1976
ISSN/ISBN: 0555-1099 PMID: 1005366 Document Number: 98121
The enzymic hydrolysis of lactose by .beta.-galactosidase [Curvularia inaequalis] (.beta.-D-galactoside-galactohydrolase, EC 3.2.1.23) immobilized in polyacrylamide gel was studied. Hydrolysis was followed by inhibition of the enzyme by both glucose and galactose. The immobilized preparation yielded 100% hydrolysis of lactose. The preparation can be used for lactose hydrolysis in milk whey in the reactor 100 cycles at 50.degree. C.