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Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli

Fong, M-Yik.; Lau, Y-Ling.; Init, I.; Jamaiah, I.; Anuar, A.Khairul.; Rahmah, N.

Southeast Asian Journal of Tropical Medicine and Public Health 34(4): 723-726

2003

ISSN/ISBN: 0125-1562
PMID: 15115078
Document Number: 552328
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

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