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Eukaryotic vectors of Celo avian adenovirus genome, carrying GFP and human IL-2 genes

Shmarov, M.M.; Cherenova, L.V.; Shashkova, E.V.; Logunov, D.Iu.; Verkhovskaia, L.V.; Kapitonov, A.V.; Neugodova, G.L.; Doronin, K.K.; Naroditskiĭ, B.S.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 2002(2): 30-35

2002

ISSN/ISBN: 0208-0613
PMID: 12180023
Document Number: 543740
Recombinant CELO avian adenoviruses carrying green fluorescent protein (GFP) and and human interleukin-2 (IL-2) genes were obtained by homologous recombination in cell culture. The resultant recombinant CELO viruses are reproduced in chick embryos in the renal tubular and chorionic allantoic membrane cells. The ability of CELO vectors to transduce human and animal cells was studied in vitro (in cell cultures) and in vivo (in laboratory animals). GFP gene delivery and expression in recombinant CELO virus in tumors in C57BL/6 mice were for the first time demonstrated for B16 melanoma. Human IL-2 gene expression and protein accumulation in allantoic fluid of chick embryos infected with CELO-IL-2 vector were detected for the first time.

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