Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors
Yan, K.; Liu, B.; Xu, M.; You, B.; Kang, N.
Wei Sheng Yan Jiu 32(3): 180-182
2003
ISSN/ISBN: 1000-8020 PMID: 12914272 Document Number: 562832
The exogenous gene cyclinD1 cDNA was inserted into eukaryotic expression vector pXJ41-neo by gene transduction and recombination techniques. The recombinant was detected by electrophoresis. Results showed that the recombinant sense plasmid (pXJ41-cyclinD1) was digested by HindIII into 229 bp, 427 bp and 7554 bp; while the recombinant antisense plasmid was digested into 427 bp, 1184 bp and 6599 bp, implying that sense and antisense pXJ41-cyclinD1 eukaryotic expression vectors were successfully recombined. The finding from this study offers an effective tool to reveal the mechanism of the carcinogenesis of environmental and occupational hazards.