Identification of pummelo (Citrus grandis (L.) Osbeck) cultivars using isozyme electrophoresis
Phan, T.Thanh.Thuy.; Nguyen, P.Dang.; Nguyen, T.Mai.Anh.
Mededelingen 67(1): 13-19
2002
ISSN/ISBN: 1373-7503 PMID: 12491965 Document Number: 541800
One hundred and one plants representing eight different cultivars of pummelo (C. grandis and esterase). Starch gel electrophoresis was used to analyse extracts from fresh young leaves collected from trees located in the provinces of Can Tho and Vinh Long in Vietnam. Isoenzyme analysis revealed that all cultivars were heterogeneous and composed of two or more clones. A total of 26 clones were discriminated from the eight cultivars. Unique combinations of isoenzyme variants of the five enzymes assayed identified 18 (69%) of the 26 clones. The remaining eight clones could be separated into four additional groups. Our results have proven the potential use of isoenzyme variability for cultivar identification and assessment of the uniformity or purity of a cultivar.