Identification, using isoenzyme electrophoresis and monoclonal antibodies, of Leishmania isolated from humans and wild animals of Ecuador
Mimori, T.; Grimaldi, G.; Kreutzer, R.D.; Gomez, E.A.; McMahon-Pratt, D.; Tesh, R.B.; Hashiguchi, Y.
American Journal of Tropical Medicine and Hygiene 40(2): 154-158
1989
ISSN/ISBN: 0002-9637 PMID: 2919726 Document Number: 345209
Six strains of Leishmania isolated from wild mammals and humans on the Pacific Coast of Ecuador were identified by isoenzyme electrophoresis and by their reactivity patterns to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. Single isolates from Sciurus vulgaris were identified as Leishmania amazonensis. Three other strains, isolated from cutaneous lesions of humans, were identified as Leishmania panamensis.AS Six strains of Leishmania isolated from wild mammals and humans on the Pacific Coast of Ecuador were identifed by isoenzyme electrophoresis and by their reactivity patterns to a cross-panel of specific MAbs using an RIA. Single isolates from Sciurus vulgaris, Potos flavus, and Tamandua tetradactyla were identified as L. mexicana amazonensis. 3 other strains, isolated from cutaneous lesions of humans, were identified as L. braziliensis panamensis.