Chiral separation of verapamil and some of its metabolites by HPLC and CE
Brandsteterová, E.; Endresz, G.; Blaschke, G.
Die Pharmazie 56(7): 536-541
2001
ISSN/ISBN: 0031-7144 PMID: 11487971 Document Number: 534806
HPLC and CE assays were developed for chiral separations of verapamil and its metabolites in serum samples. Three chiral HPLC columns (Chiralcel OJ, Chiralpak AD and Chiralcel OD-R) were tested in normal and reverse-phase modes. All HPLC analyses were performed with fluorescence detection at 276 and 310 nm. CE was realized using CM-beta-CD as a chiral selector for the enantiomeric analysis. The results of HPLC and CE studies were compared and the possibilities for the applications in therapeutic drug monitoring were discussed.