Chiral separation of 3-phenyllactic acid by capillary electrophoresis
Li, D.; Li, C.; Liu, S.; Chen, L.
Se Pu 22(3): 281-283
2004
ISSN/ISBN: 1000-8713 PMID: 15712919 Document Number: 571276
Various factors affecting the chiral resolution of 3-phenyllactic acid by capillary zone electrophoresis such as the type of cyclodextrin, concentration of the chiral selector, buffer pH, voltage, and temperature were studied and optimized. Using 30 mmol/L hydroxypropyl-beta-cyclodextrin as the chiral selector, and 0.1 mol/L phosphate solution (pH 5.5) as the running buffer, the baseline separation of 3-phenyllactic acid enantiomers was achieved with a resolution factor of 1.51. The separation voltage was 26 kV and the temperature was 25 degrees C.