Effects of amino acids and hormones on the fractional catabolic rate of albumin by the isolated perfused rat liver
Kelman, L.; Saunders, S.J.; Frith, L.; Wicht, S.
Journal of Nutrition 102(8): 1045-1048
1972
ISSN/ISBN: 0022-3166 PMID: 5051859 Document Number: 53442
Livers and blood were taken from well-fed Wistar rats for perfusion. The perfusate was of 60 ml blood, 2 ml 4.2% sodium bicarbonate, 40 mg heparin and 25 ml plasmalyte B, containing Na, Cl, K, Mg and NaHCO3, and was used alone or with added amino acids or hormones in 28 perfusions of isolated rat liver. The addition of the amino acids arginine, asparagine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan and valine at 10 times their peripheral blood values increased protein synthesis but the fractional catabolic rate of albumin was unchanged, nor was it affected by added hydrocortisone, growth hormone, triiodothyronine or glucagon but it was slightly decreased by added insulin. The albumin concentration in all perfusions was from 1.0 to 1.3 g/100 ml.