The influence of type i collagen on the cell behavior of human embryonic periosteous osteoblasts
Yang, Z.; Yu, X.; Huang, F.; Xie, H.
Hua Xi Yi Ke da Xue Xue Bao 32(1): 1-4
2001
ISSN/ISBN: 0257-7712 PMID: 12733340 Document Number: 531698
To study the influence of type I collagen (COL I) on the cell behavior of human periosteous osteoblasts (OB) and the application of type I collagen in constructing bioactive artifical bone. OB were cultured on dishes coated with bovine type I collagen in different final concentrations. The cell adhesion was examined by the methods of cell count, the proliferation of OB was studied by 3H-TdR, and the osteoblastic ability was assessed by the synthesis of collagen, osteocalcin and alkaline phosphatase (ALP). OB cultured on type I collagen layer had the following characteristics: 1. The amounts of adhesive cells were maximal top in 25 micrograms/ml final concentration; 2. The proliferation of OB was decreased above 12.5 micrograms/ml final concentration (P < 0.05); 3. The synthesis of type I collagen was reduced slightly (above 25 micrograms/ml, P < 0.05); 4. The secretion of osteocalcin was increased markedly (above 6.25 micrograms/ml, P < 0.05, which reached maximally in 25 micrograms/ml); 5. The ALP activity was also increased (above 12.5 micrograms/ml, P < 0.05). Type I collagen promotes the expression of osteoblastic phenotype and cell adhesion. When the scaffold materials for bone tissue engineering are coated with type I collagen, the osteogenesis of OB is enhanced to accelerate the transformation course from artificial bone to biological bone, the best final concentration is 25 micrograms/ml.