Correlation between changed biophysical properties of surface membranes and embryonic brain cell adhesivity. Influence of detergents, fixation, EGTA, colchicine, vinblastine, increased K+, ouabain and DMA on the primary cellular adhesivity of embryonic brain cell
Lodin, Z.; Hartman, J.; Lisá, V.; Faltin, J.; Jankásková, B.; Srajer, J.
Physiologia Bohemoslovaca 36(1): 83-88
1987
ISSN/ISBN: 0369-9463 PMID: 2954173 Document Number: 300096
Embryonic mouse brain cells were rotated for 120 min and cellular adhesivity was tested under normal conditions and in the presence of substances which change the membrane properties. A marked decrease of cellular adhesivity (but not complete inhibition) was recorded in the presence of anionic detergents, while fixation of cells caused only non-significant inhibition Colchicine (1 mumol/l) and vinblastine (10 micrograms/ml) did not significantly affect the adhesivity. Increased external K+ (10 mumol/l) and ouabain (10 mmol/l) were also without a significant effect, however, EGTA (0.1 and 0.01 mmol/l) inhibited the adhesivity significantly. 2,3 dimethyl maleinic anhydride (DMA) which removes a part of the positive charge, caused a slight decrease of adhesivity. It is suggested that the primary adhesivity of brain cells is dependent upon the structural integrity of surface membranes, while the organization of the tubular system does not play a significant role. Isotonic concentration of monovalent cations is optimal for adhesivity and an increased concentration of external K+ or ouabain did not affect adhesivity significantly.