Expression of type i collagen and its receptor system in osteoblasts
Yu, X.J.; Yang, Z.M.; Qu, Y.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 14(4): 234-236
2000
ISSN/ISBN: 1002-1892 PMID: 12078311 Document Number: 514589
To study the expression of type I collagen and its receptor system-integrin alpha 2 beta 1 in different passages of osteoblasts. The expression of type I collagen and integrin alpha 2 beta 1 in the primary, sixth and fifteenth passage of osteoblasts were detected by S-P immunohistological staining technique, and their mRNA expression by quantity RT-PCR technique. Type I collagen and integrin alpha 2 beta 1 were expressed in different passages of osteoblasts and there was no significant difference among three passages by immunohistological technique. Their mRNA expression was gradually decreased with subculture. Type I collagen promotes the adhesion and phenotype expression of osteoblasts through its receptor-integrin alpha 2 beta 1. The reductive expression of type I collagen-receptor system will decline the phenotype of osteoblasts.