Specific inhibition of vascular cell adhesion molecule-1 expression by type IV collagen in endothelial cells
Morisaki, N.; Kanzaki, T.; Tamura, K.; Saito, I.; Shiina, R.; Saito, Y.
Biochemical and Biophysical Research Communications 214(3): 1163-1167
1995
ISSN/ISBN: 0006-291X PMID: 7575525 Document Number: 449489
The effects of matrices on the expression of adhesion molecules were studied in cultured human umbilical cord vein endothelial cells(HUVEC). The expression of vascular cell adhesion molecule-1 (VCAM-1) but not of intercellular adhesion molecule-1 stimulated with various substances was remarkably low in HUVEC cultured on type IV collagen, the major component of the basement membrane, compared with that on type I collagen. This inhibition was observed not only at the protein level but also at the mRNA level. These data suggested that VCAM-1 expression in endothelial cells is strictly regulated by the underlying basement membrane.