Preparation of a Clostridium chauvoei antigen and determination of protective immunity by plate agglutination test
Claus, K.D.; Macheak, M.E.
American Journal of Veterinary Research 33(5): 1045-1052
1972
ISSN/ISBN: 0002-9645 PMID: 5023042 Document Number: 52899
Production of Clostridium chauvoei antigen is described in detail. Guinea-pigs were vaccinated at various intervals with experimental and commercial vaccines containing Cl. chauvoei antigens of various quantities and qualities. Serum agglutination titres compared favourably with the protection given to twice-vaccinated guinea-pigs which were challenged with spores at 10 to 14 days after the second vaccination. The agglutination level which consistently appeared to indicate immunity to challenge as determined by guinea-pig survival was 0.5 mu litre of less of serum to agglutinate a standardized antigen, although this did vary with the immunization period.