Protective cellular antigen of Clostridium chauvoei
Stevenson, J.R.; Stonger, K.A.
American Journal of Veterinary Research 41(4): 650-653
1980
ISSN/ISBN: 0002-9645 PMID: 7406286 Document Number: 161847
Cellular antigens of Clostridium chauvoei, strain IRP-128, were demonstrated to be important in induction of immunity against this bacterium in guinea pigs. At least one major component of the cellular antigen complex was heat-labile. Acid extraction of the bacterial cells, followed by selective purification for flagella, led to the preparation of an acid extract antigen that possessed a high degree of immunogenicity. The acid extract antigen contained flagellar components and was resolved into two major and approximately five minor protein components by polyacrylamide-gel electrophoresis.