Detection for anti-Hantavirus IgM in patient serum with silver enhanced dot immunogold filtration assay
Zhang, T.; Liu, L.; He, Z.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 14(3): 266-267
2000
ISSN/ISBN: 1003-9279 PMID: 11498694 Document Number: 524498
To establish a more rapid and simple method for detecting anti-Hantavirus IgM in patient' s serum. A silver enhanced dot immunogold filtration assay (SEDIGFA) was developed by coating the nucleocapsid protein of Hantavirus on nitrocellulose (NC) membrane, adding colloidal gold labeled sheep anti-human IgM antibody and the silver enhancer. The whole procedure can be finished within 10 minutes. The coincidence rate of the results of serum samples simultaneously operated with ELISA and SEDEIGFA was 98% for anti-Hantavirus IgM. SEDIGFA is more simple and rapid for application to small medical laboratories. It can be applied for early diagnosis of hemorrhagic fever with renal syndrome (HFRS).