Automated enhanced latex agglutination assay for rheumatoid factors in serum

Winkles, J.W.; Lunec, J.; Gray, L.; Winkles, J.W; Lunec, J; Gray, L

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Automated enhanced latex agglutination assay for rheumatoid factors in serum

Winkles, J.W.; Lunec, J.; Gray, L.

Clinical Chemistry 35(2): 303-307

1989

ISSN/ISBN: 0009-9147

PMID: 2914380

Document Number: 335912

This improved assay of rheumatoid factors in serum, described here for use with the Baker "Encore" centrifugal analyzer, is efficient, with 250-sample throughout per hour; reproducible, with between-batch CV = 5% and within-batch CV = 2% (mid-assay range); and results correlate well (r = 0.9) with those by other methods. The method is fully quantitative and automated, involves no predilution steps, and can be adapted for use in a wide range of systems. It has a sensitivity of 96% and specificity of 80% in diagnosing rheumatoid arthritis.