Verification of amplified DNA profiles by electrophoresis in various gels
Isaenko, M.V.; Ivanov, P.L.
Sudebno-Meditsinskaia Ekspertiza 43(6): 22-25
2000
ISSN/ISBN: 0039-4521 PMID: 11199073 Document Number: 523336
Differentiation of genotypical mixtures resultant from amplification analysis of mixed DNA preparations and false multi-strip profiles, which can result from amplification typing of individual DNA under certain conditions and simulate mixtures, impedes the molecular genetic identification expert evaluations. This paper offers an effective method for verification of amplification profiles of DNA, which is based on the use of alternative gel electrophoretic systems. This methodological approach helps detect the polymerase chain reaction artefacts without using additional expert material and promotes correct interpretation of expert findings even in difficult cases.