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Integration of plasmids into E. coli K12 chromosomes, caused by a Bordetella transposon

Sivov, I.G.; Beliavskiĭ, O.A.; karataev, G.I.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 2: 33-36

2000

ISSN/ISBN: 0208-0613
PMID: 10876766
Document Number: 522575
Transposition of Bordetella pertussis transposon in E. coli chromosome has been studied on a model of exclusion of donor multicopy pKK3 plasmid with coumermicin. TnBP3 induced the formation of co-integrates between the plasmid and chromosome. The structure of co-integrate was determined. Facts of exclusion of integrated structure and transposon transposition within integrated plasmid into new sites on a recipient chromosome were detected. Relationship between these processes and activity of bacterial cell recombination system has been determined.

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