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Transposon mutagenesis in Bordetella avium

Leyh, R.D.; Griffith, R.W.; Arp, L.H.

American Journal of Veterinary Research 49(5): 687-692

1988

ISSN/ISBN: 0002-9645
PMID: 2840009
Document Number: 307108
Transposon mutagenesis was used to introduce specific mutations in the chromosome of Bordetella avium. The transposon Tn5 was transferred by conjugation to B avium from Escherichia coli strain SM10 by the suicide plasmid vector pSUP1011. A defined minimal medium for growth of B avium was developed for scoring transposon-containing mutants for specific nutritional requirements. Approximately 1% of all mutants tested were auxotrophic in some form, and these organisms were grouped into 13 classes. Also, 2 classes of hemagglutination-negative mutants were isolated. Stability of Tn5 insertion was examined, and frequencies of reversion less than 10(-7) were observed, indicating stable mutations.

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