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The use of Pseudomonas sp. M plasmid pM3 for the transposon mutagenesis of Erwinia

Titok, M.A.; Prokulevich, V.A.; Maksimova, N.P.; Fomichev, I.K.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 11: 45-48

1989

ISSN/ISBN: 0208-0613
PMID: 2560815
Document Number: 332330
The transposon containing derivatives pMTF9 (Tn9), pMTF10 (Tn10) and pMTF59 (Tn5, Tn9) of the Pseudomonas sp. M conjugative plasmid pM3 demonstrating temperature-dependent instability in Erwinia cells incubated at 37 degrees C have been isolated. The obtained plasmids have been shown to be usable for transposon-mediated mutability in the bacterial cells of Erwinia generum incubated at 37 degrees C.

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