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The role of te transposition of Tn1000 from Flac+-plasmid into chromosome of Erwinia chrysanthemi in the formation of Hfr-type donors

Prokulevich, V.A.; Beliasova, N.A.; Kamara, S.F.; Kul'ba, A.M.; Fomichev, I.K.

Genetika 21(11): 1787-1793

1985

ISSN/ISBN: 0016-6758
PMID: 3000870
Document Number: 253016
Based on the data of stability of the donor state of Hfr-like strain Erwinia chrysanthemi VY1-10 in RecA+ and RecA- cells, it can be suggested that the donor properties of the strain are mediated by the presence of the genetic homology region which occurred as a result of transposition of the Tn1000 from the Flac+ plasmid into the chromosome of E. chrysanthemi. Tn1000 may be transposed into several sites on the chromosome of E. chrysanthemi ENA49. This leads to the appearance of donors transferring their chromosome from several fixed points oriT and in opposite directions. The location of these points and the direction of transfer are determined by Tn1000 insertion sites and their orientation.

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