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Studies on micronuclei induced by colchicine and cyclophosphamide using multicolor fluorescence in situ hybridization

Yang, M.; Cao, J.

Zhonghua Yu Fang Yi Xue Za Zhi 34(2): 101-103

2000

ISSN/ISBN: 0253-9624
PMID: 11860911
Document Number: 522183
To study chromosomal composition of micronuclei (MN) induced by colchicine (COL) and cyclophosphamide (CP) in mouse bone marrow erythrocytes. Multicolor fluorescence in situ hybridization (FISH) with centromeric and telomeric DNA probes was applied to analyze chromosomal composition of micronuclei induced by COL and CP in mouse bone marrow erythrocytes. About 83.5% of COL-induced MN revealed both centromeric and telomeric signals. Of the CP-induced MN, 74.5% showed telomeric signals only. Majority of COL-induced MN contain whole chromosome and that of CP-induced MN mainly contain acentric fragments. Multicolor FISH with centromeric and telomeric DNA probes was a precise technique for analyzing chromosomal composition of MN.

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Mahdy, E.; Yoshihiro, S.; Zech, L.; Wester, K.; Pan, Y.; Busch, C.; Döhner, H.; Kallioniemi, O.; Bergerheim, U.; Malmström, P.U. 1999: Comparison of comparative genomic hybridization, fluorescence in situ hybridization and flow cytometry in urinary bladder cancer Anticancer Research 19(1a): 7-12
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Multani, A.S.; Hopwood, V.L.; Pathak, S. 1996: A modified fluorescence in situ hybridization (FISH) technique Anticancer Research 16(6b): 3435-3437
Hou, J.W.; Tsai, W.Y.; Wang, T.R. 1999: Detection of KAL-1 gene deletion with fluorescence in situ hybridization Journal of the Formosan Medical Association 98(6): 448-451
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Selig, S.; Okumura, K.; Ward, D.C.; Cedar, H. 1992: Delineation of DNA replication time zones by fluorescence in situ hybridization EMBO Journal 11(3): 1217-1225
Cheng, L.; Bucana, C.D.; Wei, Q. 1996: Fluorescence in situ hybridization method for measuring transfection efficiency Biotechniques 21(3): 486-491
Van den Akker, J.; Pérot, C.; Portnoï, M.F.; Philizot, L.; Dupont, J.M.; Laporte, J.P.; Taillemite, J.L. 1995: Translocation t(6;9;8)(p23;q34;q22) in acute myeloid leukemia: Contribution of fluorescence in situ hybridization Annales de Genetique 38(3): 168-171
Tocharoentanaphol, C.; Parinayok, R.; Punthuwattana, N.; Kangwanpong, D. 2000: Fluorescence in situ hybridization: a rapid analysis to verify chromosome aberrations Journal of the Medical Association of Thailand 83 Suppl. 1: S76-S81
Virmani, A.K.; Tonk, V.S.; Gazdar, A.F. 1998: Comparison between fluorescence in situ hybridization and classical cytogenetics in human tumors Anticancer Research 18(3a): 1351-1356
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Hernández, J.M.; González, M.B.; Granada, I.; Gutiérrez, N.; Chillón, C.; Ramos, F.; Ribera, J.M.; González, M.; Feliu, E.; San Miguel, J. 2000: Detection of inv(16) and t(16;16) by fluorescence in situ hybridization in acute myeloid leukemia M4Eo Haematologica 85(5): 481-485
Dupont, J.M.; Lebbar, A.; Dupuy, O.; Frydman, N.; Letessier, D.; Auvinet, P.; Rabineau, D. 1998: Polymerase Chain reaction generated probes for fluorescence in situ hybridization Morphologie: Bulletin de l'Association des Anatomistes 82(257): 21-24
Fiche, M. 2001: Fluorescence in situ hybridization (FISH): current developments and prospects in pathology Annales de Pathologie 21(5): 383-385
Aribarg, A.; Ngeamvijawat, J.; Chanprasit, Y. 2000: Investigation of sex chromosome abnormalities in teratozoospermia of infertile men using fluorescence in situ hybridization Journal of the Medical Association of Thailand 83(7): 737-742
Liu, J.; Wang, Z-Min.; Zhen, S-Fang.; Wu, X-Peng.; Ma, D-Xin.; Li, Z-Hui.; Liu, B.; Zhao, Z-Lun.; Ke, Y. 2004: Aberration of X chromosome in liver neoplasm detected by fluorescence in situ hybridization Hepatobiliary and Pancreatic Diseases International: Hbpd Int 3(1): 110-114
Zhao, L.; Khan, Z.; Hayes, K.J.; Glassman, A.B. 1998: Interphase fluorescence in situ hybridization analysis: a study using centromeric probes 7, 8, and 12 Annals of Clinical and Laboratory Science 28(1): 51-56
Dinchuk, J.E.; Kelley, K.; Boyle, A.L. 1994: Fluorescence in situ hybridization of interphase nuclei isolated from whole blood of transgenic mice Biotechniques 17(5): 954-961