Express Fluorescence in Situ Hybridization Methods for the Detection of Staphylococcus Aureus
Lawson, T.S.; Connally, R.E.; Vemulpad, S.; Piper, J.A.
Clinical Laboratory 57(9-10): 789-794
2011
ISSN/ISBN: 1433-6510 PMID: 22029198 Document Number: 12568
Background: As a proof-of-concept, the feasibility of detecting Staphylococcus aureus faster than previous whole-cell fluorescent in situ hybridization (FISH) methods was tested. Methods: Isolates of Staphylococcus were treated with three rapid slide-based FISH protocols and DNA probes. Protocols were shortened by optimizing, combining or omitting steps. Results: All FISH protocols detected S. aureus and not the phenotypically similar Staphylococcus epidermidis . The express FISH assay was completed in 24 minutes. The one-step FISH assay with NaCl and the one-step with phosphate buffered saline (PBS) assay took 19 minutes, but yielded a weaker signal. Conclusions: : The exploratory study identified S. aureus two to three times faster than previous methods. Additional testing in a clinical laboratory scenario (for example with positive blood-culture bottles) is warranted.
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