Localization of proteins, immunologically similar to Eschericha coli RecA protein, in structures of cultured cells of Hela and K-562 cell lines
Loseva, E.F.; Il'inskaia, L.E.; Kalinin, V.V.
Genetika 36(12): 1614-1621
2000
ISSN/ISBN: 0016-6758 PMID: 11190468 Document Number: 520346
Earlier, polyclonal antibodies to the Escherichia coli RecA protein revealed immunologically related proteins only in the reproductive tissues of various eukaryotes and the proteins were localized to the various structures of the synaptonemal complex (SC). Indirect immunofluorescent FITC staining with polyclonal antibodies against RecA detected RecA-like antigens in the cell center (centrosome) and the replicated centrioles of fixed HeLa and K-562 cells. Human and mouse genes, which were previously cloned by immunological relatedness of their products to E. coli RecA, were used as hybridization probes in Northern blot analysis of the transcription activity of similar genes in K-562 cells. A gene homologous to the cloned mouse gene proved transcribed in the cells studied.