Reconstruction of humoral response to sperm antigens in scid mice
Grygielska, B.; Fiszer, D.; Domagała, A.; Kurpisz, M.
Ginekologia Polska 71(6): 577-585
2000
ISSN/ISBN: 0017-0011 PMID: 11002564 Document Number: 515167
Production of specific human antisperm antibodies by using human-SCID mice model with deposited peripheral blood lymphocytes. Human peripheral blood lymphocytes (PBL's; CD8(+)-negative cell fraction) were grafted to the peritoneal cavity of severely-combined immunodeficient (SCID) mice at concentration of 20-35 x 10(6) cells per mouse. Lymphocytes were obtained from non-sensitized individual (to sperm antigen) and from in vivo primed males (vasectomized). Two sets of experiments were carried out, with 'native' (glycosylated) and enzymatically deglycosylated sperm antigenic extracts. In all applied variants, sperm antigens were administered with Complete and then with Incomplete Freund adjuvant to improve an immune response. This approach allowed us to obtain better pronounced humoral antisperm response, specific to sperm deglycosylated antigens when PBL's were obtained from individuals in vivo sensitized to sperm (after vasectomy).