Reconstitution of Hu-SCID mice transplanted with human tonsillar lymphocytes immunized with dengue virus-2 in vitro and its immune response
Zuo, L.; Guo, H.; Zhang, C.
Zhonghua Yi Xue Za Zhi 76(8): 597-599
1996
ISSN/ISBN: 0376-2491 PMID: 9275474 Document Number: 455713
Human-TLC-SCID mice were constructed by intraperitoneal (i.p.)transfer of human tonsillar lymphocytes (TLC) immunized with dengue virus-2(DV2) in vitro, then boostered with different doses of DV 2 antigen in vivo. The results showed that human immunoglobulin (Ig) and specific human antiDV2 antibodies could be detected in sera of the hu-TLC-SCID mice. Two weeks after transplantation of the in vitro immunized hu-TLC,the highest human IgG concentration was as high as 412. 8 mu-g/ml. The SCID mice transplanted with 1.5 times 10-8 human TLC immunized in vitro were given i.p. booster injections with 0.5 ml of different concentrations of inactivated DV2. The first injection was given 1 day after the transplantation of human TLC, and the injection were repeated with 2-WK intervals. Two and four weeks later, specific human anti-DV 2 IgM or IgG could be detected in these sera and the highest titer reached 1:6400. The levels of human IgG and specific human anti-DV2 antibodies were related with the does of DV2 antigen.