Analysis of candidate human blood stem cells in "humanized" immune-deficiency SCID mice
Péault, B.; Weissman, I.; Baum, C.
Leukemia 7(Suppl 2): S98-101
1993
ISSN/ISBN: 0887-6924 PMID: 7689676 Document Number: 409716
Immune deficient SCID mice support the hematopoietic development of surgically implanted human fetal thymus and bone. The usability of the resulting SCID-hu hematolymphoid chimaera as an assay system for human candidate blood stem cells was suggested by the observation that allogeneic CD34+ cells sorted from fetal liver and bone marrow can engraft and differentiate in the transplanted human thymus and bone marrow. The SCID-hu mouse has been further used, in combination with a mouse bone marrow stromal cell line that supports human myeloid and B lymphoid differentiation in vitro, to identify a minor subset of fetal CD34-expressing cells that exhibit multilineage hematopoietic potentialities.