Studies on method for the determination of kaerophyllin in Bupleurum smithii wolff Wolff var. parvifolium
Dou, H.S.; Zeng, S.X.; Wang, Y.H.; Shang, M.Y.
Zhongguo Zhong Yao Za Zhi 25(8): 488-489
2000
ISSN/ISBN: 1001-5302 PMID: 12515213 Document Number: 512486
To establish a quantitative method for the determination of kaerophyllin in Chinese medicine Bupleurum smithii ver. parvifolium. Kaerophyllin was determined by HPLC, the mobile phase consisted of methanol-water(70:30), the flow rate was 1 ml.min-1, the eluate was monitored with UV detector at 328 nm. The average recovery was 97.1% with RSD = 2.15 (n = 5). The result was precise and stable. This method was sensitive and its repeatability was excellent. This method may serve determination of kaerophyllin in B. smithii var. parvifolium.