Effect of pH on Ki-67 antigen retrieval in renal cell carcinoma
Masera, A.; Stanisa, O.; Ovcak, Z.; Cör, A.
Acta Medica Croatica Casopis Hravatske Akademije Medicinskih Znanosti 53(1): 15-17
1999
ISSN/ISBN: 1330-0164 PMID: 10437272 Document Number: 511292
The efficacy of Ki-67 antigen retrieval (AR) was assessed in 20 formalin fixed-paraffin embedded specimens of renal cell carcinoma using three solutions with different pH values. The AR was performed by means of microwave oven heating up to 97 degrees C for 15 minutes. Better results were obtained using solutions with pH values 8.0 and 9.9 compared with AR in a solution of pH 6.0, which is routinely used in daily pathological practice. Individual testing of various AR conditions is recommended to obtain the best immunohistochemical results.