Proliferating cell nuclear antigen (PCNA) as a prognostic factor in renal cell carcinoma
Morell-Quadreny, L.; Clar-Blanch, F.; Fenollosa-Enterna, B.; Perez-Bacete, M.; Martinez-Lorente, A.; Llombart-Bosch, A.
Anticancer Research 18(1b): 677-682
1998
ISSN/ISBN: 0250-7005 PMID: 9584051 Document Number: 492965
Background: A retrospective study was performed on patients with renal cell carcinoma to determine whether tumoral proliferating cell nuclear antigen (PCNA) is a predictive factor. Methods: We studied immunohistochemical PCNA expression with pcl0 monoclonal antibody in 109 renal tumor paraffin sections. These tumors were previously classified according to cellular type by Thoenes, Furman's grading and Robson's staging. Moreover, we counted the number of mitoses in 10 high power fields (HPF) and also evaluated the tumoral necrosis percentage. Ten year survival curve of Kaplan and Meier was obtained for 90 patients. Results: Nuclear immunostaining for PCNA showed a statistical correlation with Robson's stage, cellular type and nuclear grade. Moreover, the number of positive nuclei was higher in tumors presenting an elevated mitosis count and higher degree of necrosis. Survival way significantly poorer in patients whose PCNA index was greater than 5%. Nuclear PCNA immunostaining was shown to be an independent prognostic factor in patients with Robson stage I and also in those who had high cytological grading. Conclusions: These results show PCNA to be a prognostic marker for RCC.