Are renal cell carcinoma cells able to modulate the cytotoxic effect of tumor infiltrating lymphocytes by secretion of interleukin-6?
Steiner, T.; Junker, U.; Wunderlich, H.; Schubert, J.
Anticancer Research 19(2c): 1533-1536
1999
ISSN/ISBN: 0250-7005 PMID: 10365139 Document Number: 511106
Background: Interleukin(IL)-6 can suppress the cisplatin-induced induction of apoptosis in renal cell carcinoma (RCC) in vitro. There are reports on IL-6 secretion by tumor cells. Materials and Methods: In supematants of RCC cultures we measured IL-6 levels by a bioassay. In a videomicroscopic microcytotoxicity assay the immunomodulating effect of secreted cytokines on the cytotoxicity of tumor infiltrating lymphocytes (TIL) was studied. Results: The IL-6 concentrations in 17 supematants of healthy renal cells tended to be higher than in the supernatants of tumor cell cultures. By immunostaining we detected IL-6 in 3/6 cell cultures from healthy renal tissue, in 12/22 tumor tissue samples, and in 8/18 tumor cultures respectively. Supernatants suppressing cytotoxic activity of TIL showed higher IL-6 concentrations in tendency. Suppression of cytotoxicity could also be induced by adding discrete concentrations of IL-6. Conclusions: IL6 secreted by tumor cells seems to be tumor protectingagainst cytotoxic TIL. Blocking this mechanism could be an additional approach in immunotherapy of RCC.