Interaction between gene p53 and oncogene mdm2 in human glandular lung cancer cell line GLC-82
Li, P.; Rao, G.; Chen, Y.; Ding, Z.; Yu, W.
Chinese Medical Journal 112(9): 850-853
1999
ISSN/ISBN: 0366-6999 PMID: 11717960 Document Number: 509858
To study the interaction between oncogene mdm2 and wp53 in human glandular lung cancer cell line GLC-82. By lipofectamine-mediated DNA transfection, wp53 and mdm2 were transfected separately or co-transfected into GLC-82 cells via retrovival vector pDOR-neo, a carrier of wp53 and mdm2. The growth of GLC-82 cells was blocked and their DNA synthesis inhibited by wp53, its colony-forming rate in soft agar culture and the tumorigenicity in nude mice declined and mdm2 antagonized the function of wp53. After the recombinant vector pDOR-mdm2 was transfected into GLC-82 cells containing wp53, mdm2 partially deprives wp53 of its function of inhibiting the growth of GLC-82 cells.