Chromosomal localization of transforming oncogene BGC-Ha-ras in gastric cancer cell line
Hou, Y.
Zhonghua Zhong Liu Za Zhi 12(2): 95-97
1990
ISSN/ISBN: 0253-3766 PMID: 2205454 Document Number: 356910
BGC-Ha-ras, isolated from the human gastric cancer cell line BGC-823 and activated by point mutation, is a transforming oncogene. To study whether this oncogene can still be located in the original position of chromosome 11 of BGC-823 cell line, chromosomal in situ hybridization method was used. The results showed that 31% silver grains were localized on chromosome 11, 83% of which on the 11p15 region, and in addition, 10% grains on distal end of the short arm of the other abnormal chromosome. Such results are significant in further study of the relation between gene localization and carcinogenesis.