Acute reduction in whole cell conductance in anoxic turtle brain
Ghai, H.S.; Buck, L.T.
American Journal of Physiology 277(3): R887-R893
1999
ISSN/ISBN: 0002-9513 PMID: 10484508 Document Number: 505963
We tested the effect of anoxia, a "mimic" turtle artificial cerebrospinal fluid (aCSF) consisting of high Ca2+ and Mg2+ concentrations and low pH and adenosine perfusions, on whole cell conductance (Gw) in turtle brain slices using a whole cell voltage-clamp technique. With EGTA in the recording electrode, anoxic or adenosine perfusions did not change Gw significantly (values range between 2.15 ± 0.24 and 3.24 ± 0.56 nS). However, perfusion with normoxic or anoxic mimic aCSF significantly decreased Gw. High that result in acute changes in Gw with the onset of anoxia. Reprinted by permission of the publisher.