Cell culture procedure and the optometric method to study cells in vitro
Hanu, N.; Răchită, M.
Roumanian Archives of Microbiology and Immunology 58(2): 147-155
1999
ISSN/ISBN: 1222-3891 PMID: 11845453 Document Number: 498652
The cells are cultivated using an original licensed procedure named "homogeneous sets". This consists of cell growth in monolayer using as holder several plates specially designed to enable the study both by use of the optometric method we elaborated and by classical techniques (microscopy). Using the suggested method, a stress can be induced under control. Stress induction is undertaken through physical, chemical, biological factors or all of these taken together. The reactivity to stress consists of changes in cell morphology, which can be evidenced by graphically recording the optical density variation in time, contraction curves being thus obtained. Dimensions, the initial and final values, the characteristic zones, angles and the arrow are thus chosen as to characterize the cell reactivity and the most significant periods of time during the experiment. The contraction curves can be interpreted somewhat similarly to the electrocardiogram, so that the term we chose is the "OPTOGRAM". The recovery to the cells normal state is ascertained by the relaxation curves. The cell culture procedure and the optometric evaluation method provide the means to study cell reactivity to different aggressive or protective agents.