Giant cell tumor of bone: analysis of proliferative cells by double-labeling immunohistochemistry with anti-proliferating cell nuclear antigen antibody and culture procedure

Abe, Y.; Yonemura, K.; Nishida, K.; Takagi, K.

Nihon Seikeigeka Gakkai Zasshi 68(5): 407-414

1994


ISSN/ISBN: 0021-5325
PMID: 7914219
Document Number: 432226
Giant cell tumor of bone (GCT) consists of multinucleated giant cells, fibroblast-like cells, and macrophage-like cells, but what constitutes the principal neoplastic cells and their histogenesis remains controversial. Proliferating cells in GCT have been immunophenotypically characterized with the cell cycle-related antigen proliferating cell nuclear antigen (PCNA). In seven cases, PCNA monoclonal antibody was applied by double-labeling immunohistochemical methods together with other cell-specific monoclonal antibodies. Many proliferating PCNA-positive cells were found in the vimentin-positive fibroblast-like cells, while almost all macrophage-like cells were negative for PCNA. The study of cultured cells showed that the fibroblast-like spindle-shaped cells were a neoplastic element. In addition, osteoclast-like cells were detected in co-culture systems of cells cultured from GCT with spleen cells. These studies support the concept that GCT is seen as a neoplasm of fibroblast-like cells with a strong reactive infiltration of cells from the mononuclear phagocyte system.

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