Expression of proliferating cell nuclear antigen in Wilms' tumor
Chen, F.; Liu, G.; Zhang, Z.; Li, Z.; Cai, W.
Chinese Medical Journal 110(5): 352-353
1997
ISSN/ISBN: 0366-6999 PMID: 9594301 Document Number: 471454
Objective: To investigate the expression of proliferating cell nuclear antigen (PCNA) and its clinical significance in Wilms' tumor. Methods: PCNA immunoreactivity on paraffin-embedded tissues was assessed retrospectively in 35 cases using labelled streptaridin biotin (LSAB) procedure, and its association with clinical and other biological features was studied. Results: Twelve tumors (34.29%) expressed PCNA. Expression rate increased with the advance of stage (P lt 0.05). The S-phase fraction and proliferation index (PI) in children with PCNA positive expression were higher than those in children with negative PCNA expression (P lt 0.05). The survival rate (16.67%) in patients with positive PCNA expression was significantly lower than that (73.91%) in no-expression ones (P lt 0.01). Conclusion: PCNA is a sensitive indicator for cell proliferation and is clinically useful in identifying a poor prognostic group of patients with Wilms' tumor.