Modification of a method to study cell fusion induced in vitro by viruses
De Micco, P.; Tamalet, J.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 173(1): 110-116
1979
ISSN/ISBN: 0037-9026 PMID: 226237 Document Number: 139355
A studying method of viruses induced cell-fusion is described, using monolayers of Vero monkey cells. Nature and density of cells and infection multiplicity are studied. The sensibility is equal to 4 hemagglutining units of Sendaï and accuracy about +/- 3 % of polykaryocytosis. Quantification. comparison and kinetics study of cell-fusion are allowed by this method which may lead to systematic and routine exploration of cell-fusion ability of biological extracts from slow degenerative diseases of the human central nervous system.