Top Abstract Purchase Related
Home
>
Node
>
Subnode

In vivo and in vitro experimental study on the effect of fluoride-induced autophagy in rat HAT-7 cell line

Lei, S.; Zhang, Y.; Zhang, K.-Q.; Li, J.

Shanghai Kou Qiang Yi Xue 25(4): 426-430

2016

ISSN/ISBN: 1006-7248
PMID: 27858064
Document Number: 690740
To study the effect of fluoride on autophagy in rat ameloblasts both in vitro and in vivo. Logarithmic-phase HAT-7 cells were cultured in different concentrations of fluoride for 48h. Transmission electron microscopy (TEM) was used to detect autophagosomes. Western blot and RT-qPCR were carried out to examine the expression of LC3 and Beclin 1. Forty Wistar rats were divided into 4 groups randomly. The expression of LC3 and Beclin 1 in rats was investigated by immunohistochemical staining in vivo. The data were analysed using SPSS13.0 software package. The amount of autophagosomes in the experimental group was significantly more than that in the control group (P<0.05). The expression of LC3 and Beclin 1 were up-regulated in dose dependent manner after treatment with fluoride. Regression analysis showed that fluoride dependently induced the expression of LC3 and Beclin 1 (P<0.05).Immunohistochemical analysis revealed that the expression of LC3 and Beclin 1 in rat ameloblasts in the experimental groups was positive compared to control group. Excessive fluoride induced autophagy in ameloblasts both in vitro and in vivo.

Document emailed within 1 workday
Secure & encrypted payments

Chaet, R.; Wei, S.H. 1977: The effect of fluoride impregnated dental floss on enamel fluoride uptake in vitro and streptococcus mutans colonization in vivo Asdc Journal of Dentistry for Children 44(2): 122-126
Tosi, P.; Calabresi, P.; Goulette, F.A.; Renaud, C.A.; Darnowski, J.W. 1992: Azidothymidine-induced cytotoxicity and incorporation into DNA in the human colon tumor cell line HCT-8 is enhanced by methotrexate in vitro and in vivo Cancer Research 52(15): 4069-4073
Jing, Y.-H.; Zhang, L.; Gao, L.-P.; Qi, C.-C.; Lv, D.-D.; Song, Y.-F.; Yin, J.; Wang, D.-G. 2017: Autophagy plays beneficial effect on diabetic encephalopathy in type 2 diabetes: studies in vivo and in vitro Neuro Endocrinology Letters 38(1): 27-37
Cai, S.; Zhou, Y.; Liu, J.; Liu, D.; Jiang, R.; Wang, Y. 2012: Experimental study on human leukemia cell line K562 senescence induced by ginsenoside Rg1 Zhongguo Zhong Yao Za Zhi 37(16): 2424-2428
Plempel, M.; Bartmann, K. 1972: Experimental study of the antimycotic effect of clotrimazole in vitro and in vivo when administered locally Arzneimittel-Forschung 22(8): 1280-1289
Ducore, J.M.; McNamara, L. 1986: Effect of alpha-difluoromethylornithine on L-phenylalanine mustard-induced cytotoxicity and DNA interstrand cross-linking in a human cell line in vitro Cancer Research 46(3): 1068-1072
Li, H.F.; Huang, L.F.; Chen, L.H. 2019: Chitooligosaccharides inhibit A549 lung cancer cell line proliferation by regulating cell autophagy Journal of Biological Regulators and Homeostatic Agents 33(5): 1527-1532
Habib, F.A.; Lobocki, C.; Ezhuthachan, R.; Chelladurai, M.; Preventza, O.; Mittal, V.K. 2001: Interferon alpha2b inhibits the murine melanoma cell line Cloudman S91 in vivo but not in vitro: a model for studying tumor cell-cytokine interactions American Surgeon 67(3): 257-260
Zhang, J.B.; Ge, M.; Zhang, Z.X. 1980: In vitro study of synergic effect of hyperthermia and camptothecine on esophageal cancer cells (109 cell line) Zhonghua Zhong Liu Za Zhi 2(1): 42-44
Leelavinothan, P.; Kalist, S. 2011: Beneficial effect of hesperetin on cadmium induced oxidative stress in rats: an in vivo and in vitro study European Review for Medical and Pharmacological Sciences 15(9): 992-1002
Bernal, S.; Thompson, R.; Gilbert, F.; Baylin, S.B. 1983: In vitro and in vivo growth characteristics of two different cell populations in an established line of human neuroblastoma Cancer Research 43(3): 1256-1260
Nishihara, K.; Barth, R.F.; Wilkie, N.; Lang, J.C.; Oda, Y.; Kikuchi, H.; Everson, M.P.; Lotze, M.T. 1995: Increased in vitro and in vivo tumoricidal activity of a macrophage cell line genetically engineered to express IFN-gamma, IL-4, IL-6, or TNF-alpha Cancer Gene Therapy 2(2): 113-124
Cohen, O.; Einav, Y.; Cohen, Y.; Karasik, A.; Ferber, S. 2000: Implantation of rat insulinoma cell line into cyclosporine treated rats. Effect of the in vivo environment on beta-cell specific gene expression Transplantation 69(7): 1464-1470
Sugimoto, T.; Tatsumi, E.; Takeda, K.; Minato, K.; Sagawa, K.; Minowada, J. 1984: Modulation of cell surface antigens induced by 12-O-tetradecanoyl-phorbol 13-acetate in two myeloblastic cell lines, a promyelocytic cell line, and a monoblastic cell line: detection with five monoclonal antibodies Journal of the National Cancer Institute 72(4): 923-928
Chen, Z.; Luo, Z.; Yang, S. 2001: Comparison of the effect of fluoride and non-fluoride dentifrices on enamel demineralization and remineralization in vitro Hua Xi Kou Qiang Yi Xue Za Zhi 19(1): 20-22
Tatsumi, K.; Nakamura, T.; Wakisaka, G. 1974: Damage of mammalian cell DNA in vivo and in vitro induced by neocarzinostatin Gan 65(5): 459-461
Masucci, J.J.A.; Caldwell, G.G.W.; Jones, W.W.J.; Juzwin, S.S.J.; Sasso, P.P.J.; Evangelisto, M. 2001: The use of on-line and off-line chromatographic extraction techniques coupled with mass spectrometry for support of in vivo and in vitro assays in drug discovery Current Topics in Medicinal Chemistry 1(5): 463-471
Hackenberg, R.; Loos, S.; Nia, A.H.; Kunzmann, R.; Schulz, K.D. 1998: Expression of placental protein 14 by the new endometrial cancer cell line MFE-280 in vitro and by endometrial carcinomas in vivo Anticancer Research 18(2a): 1153-1158
Kaufmann, Y.; Berke, G. 1981: Cell surface glycoproteins of cytotoxic T lymphocytes induced in vivo and in vitro Journal of Immunology 126(4): 1443-1446
Tandon, S.; Paul, S.T.; Kiran, M. 1995: Effect of low fluoride dentifrices on microhardness of sound enamel. in vitro study Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research 6(4): 117-122