Molecular cloning of a novel human lung cancer-associated antigen cDNA
Cao, Z.; Fan, M.
Zhonghua Zhong Liu Za Zhi 20(2): 122-124
1998
ISSN/ISBN: 0253-3766 PMID: 10920961 Document Number: 495404
To clone the gene of human lung cancer-associated antigen. The human lung squamous carcinoma cell line L-78 cDNA expression library was constructed with lambda gt 11 Sfi-Not directional cloning vector and screened with the anti-human lung cancer McAb ALT-04. The cDNA clone named hlc-14 was sequenced and further studied. NIH3T3 cells were transfected with the recombinant pXJ41-hlc14 plasmid, and the expression of hlc-14 cDNA was detected and characterized immunohistochemically. Soft agar colony formation assay of the transfectants was performed. A human lung cancer lambda gt 11 cDNA library of 1.4 x 10(6) pfu/ml was constructed. Six cDNA clones were isolated following 4 rounds of immunoscreening. The largest cDNA clone hlc-14 contained 954 bp with no homology to any known gene when screened through GenBank. In the NIH3T3 cells transfectant of hlc-14 cDNA, the expression of the lung cancer-associated antigen was demonstrated. The ability of colony formation of the transfectant in the soft agar was enhanced. The hlc-14 clone is a novel human lung cancer-associated antigen cDNA. It provides clues for the study of carcinogenesis mechanism of lung cancer and a novel target gene for the gene therapy of lung cancer.