Outbreak of viral meningoencephalitis. Comparison of 2 biological systems used for isolation of the virus
Abreu Nicot, I.M.; Bello Corredor, M.; Más Lago, P.; Arteaga Hernández, E.; Avalos Redón, I.; Sarmiento Pérez, L.R.; Palomera Puente, R.; Pérez Santos, L.
Revista Cubana de Medicina Tropical 50(1): 71-74
1998
ISSN/ISBN: 0375-0760 PMID: 9842272 Document Number: 492807
An increase of the cases of viral meningoencephalitis was reported in our country from October to December, 1995. 23 faeces specimens were received from 2 provinces aimed at obtaining the viral isolation by their inoculation in cell culture and in newborn mice at the same time in order to compare the sensitivity of both systems in isolation. A higher number of isolations was obtained in cell culture. The cell culture from kidney of a normal adult green African monkey (Vero) proved to be more sensitive. 18 isolations were obtained and identified as Coxsackievirus B5. An anatomical and pathological study was conducted in inoculated mice and injuries compatible with the enteroviral infection were observed.