Diagnosis of cytomegalovirus infections in patients with HIV infection. Comparison of the indirect immunofluorescence and viral isolation techniques
Valdivia, A.; Alvarez, M.; Marrero, M.; Valdés, O.; Muné, M.
Revista Cubana de Medicina Tropical 44(3): 212-215
1992
ISSN/ISBN: 0375-0760 PMID: 9768219 Document Number: 390213
The technique for the detection of fluorescent early antigen was compared to the classical method of viral isolation in cells from the human lung. The study was performed with the use of 85 urine samples from 64 patients presenting with HIV infection. The technique for the detection of fluorescent early antigens showed a sensitivity of 91%, an specificity of 97%, and a coincidence of 94% with respect to viral isolation. The main advantage of the technique for the detection of fluorescent early antigens with respect to viral isolation is that the former provides a quicker diagnosis of cytomegalovirus infection within 48-72 hours, besides being easy to perform.