Etiologic differentiation in lower respiratory tract virus infections by indirect immunofluorescence
Qiu Fuxi; Zhang Haoyan; Zheng Qijing; Shao Lan; Wang Xiuyun; Li Guiying; Suo Yuqin
Chinese Medical Journal 92(9): 605-612
1979
ISSN/ISBN: 0366-6999 PMID: 227647 Document Number: 149651
Of the 50 young children diagnosed clinically as having virus pneumonia, 24 (48%) had type 3 or 7 adenovirus antigen [Ag] detected in exfoliated nasopharyngeal cells and 2 of them had the same viral Ag in the lung tissue which yielded type 7 adenovirus. Respiratory syncytial virus [RSV] Ag was detected in 5 (10%). Type I, II or III parainfluenza virus Ag was detected in 3 (6%) and the same viral Ag was detected in 1 of them in the lung tissue which yielded type I parainfluenza virus. Of the 7 infants diagnosed clinically as bronchiolitis, 3 had type 3 or 7 adenovirus Ag detected in exfoliated nasopharyngeal cells and 2 had RSV Ag. Type I, II or III parainfluenza virus Ag was detected in exfoliated nasopharyngeal cells in 1 child diagnosed clinically as having pleurisy. In 11 children with diseases other than respiratory tract infections, the results of detection of these 3 kinds of viral Ag were all negative. Of the 39 cases of virus pneumonia, a 4-fold or greater rise in hemagglutination-inhibiting antibody [Ab] titer to types 7 and 3 adenoviruses was demonstrated in 39.5% and 17.9%, totaling 53.8%; a 4-fold or more increase in neutralizing Ab titer to RSV and a 4-fold or more increase in hemadsorption-inhibiting Ab titer to type I parainfluenza virus were shown in 10.3% each. Of the 5 cases of bronchiolitis, 4-fold or greater rises were shown in Ab titers, 1 case to type 7 adenovirus, 1 to type 3 adenovirus and 2 to RSV. No elevation of Ab titers to these 3 kinds of viruses was observed in the 10 children with diseases other than respiratory tract infections. There was good correspondence between the detection of viral Ag by indirect immunofluorescence and Ab titer assay in paired sera.