Functional properties of hepatic T-lymphocytes from mice immunized with an alloantigen in vivo
Kazanskiĭ, D.B.; Chernysheva, A.D.; Sernova, N.V.; Abronina, I.F.; Anosova, N.G.; Rodin, D.V.; Pobezinskiĭ, L.A.; Agafonova, E.L.
Molekuliarnaia Biologiia 32(6): 1036-1043
1998
ISSN/ISBN: 0026-8984 PMID: 9929883 Document Number: 491212
Mature T-lymphocytes accumulate in the liver as a result of deletion induced by in vivo administration of antigen peptide; surface expression of T-cell receptors and coreceptor molecules decreases and as result they die according to apoptosis mechanism. It was shown that in mice primed with allogenic tumor cells, the fraction of functional effector cytotoxic T-lymphocytes (CTL) was 2-4 times higher in the population of liver mononuclears than in spleen mononuclears. Fluorescence activated cell sorter (FACS) of liver mononuclears demonstrated a double increase of the portion of CD8+ cells and a decrease of the portion of CD4+ and CD3+CD4-CD8- cells. Long-lived memory cells were found in both organs 2 month after the immunization. These cells were capable of antigen-specific proliferation in vitro in response to the priming antigen. FACS analysis did not show significant differences between populations of naive and alloantigen-primed T-lymphocytes. The results of the study confirmed direct participation of the liver in allogenic immune response in vivo. A model was proposed according to which the liver carried out the selection of memory T-cells with high affinity of T-cell receptors.