Restriction endonuclease Sse9I (correction of Sse91) from Sporosarcina strain SP.9D recognizes the 5&apos;-AATT-3&apos; DNA sequence

Gonchar, D.A.; Dedkov, V.S.; Verkhozina, V.A.; Kusner, I.S.; Shevchenko, A.V.; Degtiarev, S.Kh.; Gonchar, D.A; Dedkov, V.S; Verkhozina, V.A; Kusner, I.S; Shevchenko, A.V; Degtiarev, S.Kh

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Restriction endonuclease Sse9I (correction of Sse91) from Sporosarcina strain SP.9D recognizes the 5'-AATT-3' DNA sequence

Gonchar, D.A.; Dedkov, V.S.; Verkhozina, V.A.; Kusner, I.S.; Shevchenko, A.V.; Degtiarev, S.Kh.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1998(1): 32-34

1998

ISSN/ISBN: 0208-0613

PMID: 9511140

Document Number: 488589

Sse91, a type II restriction endonuclease, has been isolated from Sporosarcina species 9D. The recognition sequence and cleavage point of restriction endonuclease Sse91 have been determined as 5'-decrease AATT-3'. The new enzyme is an isoschizomer of Tsp5091, but its optimal incubation temperature is 55 degrees C and it is inactivated at 65 degrees C for 20 min.