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Identification of leptospiral isolates by bacterial restriction endonuclease analysis (Brenda)

Venkatesha, M.D.; Ramadass, P.

Indian Journal of Medical Microbiology 19(2): 26-29

2001

ISSN/ISBN: 0255-0857
PMID: 17664802
Document Number: 527886
DNA samples from 19 reference serovars belonging to 19 different serogroups of Leptospira interrogans and two serovars belonging to Leptospira biflexa were examined by bacterial restriction endonuclease analysis using EcoR I and Hae III enzymes. All the serovars gave unique restriction patterns that differed from each other. DNA from 10 local isolates digested with these enzymes produced patterns which on comparison with the standard patterns produced by reference strains could be identified to serovar level.

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