TLC analysis of harpagoside in devil claw extract and its tablets
Qian, H.; Xie, P.
Zhongguo Zhong Yao Za Zhi 23(12): 726-729; 764
1998
ISSN/ISBN: 1001-5302 PMID: 12242821 Document Number: 488345
To set up a method for TLC identification of the Devil Claw Extract and its tablets, and make a quantitative analysis of harpagoside the main active constituent in the Extract. A characteristic TLC chromatogram was obtained by using pre-coated silica gel 60F254 plate, chloroform-ethyl acetate-methanol-water as the solvent system and 5% vanillin-sulfuric acid solution or 10% sulfuric acid-ethanol solution as the spray reagent; and quantitative analysis was carried out using silica gel plate modified by 1% sodium hydroxide, and UV absorptive scanning at 283 nm. The regression equation is Y = 20.33X + 92.72, r = 0.9993, linear range between 16.8-151.2 ng, and average recovery 101.2%. This method has been validated by linearity, stability, sensitivity and reproducibility tests, and proved to be accordant with the requirement for quantitative analysis of the herbal medicine in question.